Laboratoire Interdisciplinaire des Energies de Demain
Présentation
Le laboratoire en quelques mots
Le Laboratoire Interdisciplinaire des Energies de Demain (LIED) a pour objectif fondateur de développer « l’écologie des énergies », en menant à la fois recherches scientifiques et techniques guidées par les problèmes à résoudre dans le cadre de la transition énergétique et de l’accroissement des besoins mondiaux en énergie.
Il répond à la nécessité d’une approche interdisciplinaire des problèmes posés, en fédérant les membres – universitaires ou venant d’entreprises – des secteurs Sciences (Biologie, Chimie, Informatique, Mathématiques, Physique, Sciences de l’Ingénieur, Sciences de la Terre) et Sciences Humaines et Sociales (Anthropologie, Economie, Géographie, Histoire, Philosophie, Ecologie, Sciences politiques, Sociologie).
Les membres de l’UMR LIED (17 du secteur sciences et 9 du secteur SHS) se composent de 8 PR, 11 MCU, 3 personnels CNRS (1CR, 1IR, 1assimilée) et de 4 consultants. Le LIED constitue également la pierre angulaire de l’Institut des Energies de Demain (IED), appelé à devenir l’un des quatre instituts transversaux de l’IDEX SCP. Celui-ci comprend l’UMR LIED, la fédération IED et le réseau international PIERI (Paris Interdisciplinary Energy Research Institute).
L’ensemble forme un « dispositif » en cinq modes d’appartenance dont le premier est l'UMR LIED proprement dite. Le deuxième mode d’appartenance à LIED rassemble des membres de la plupart des laboratoires du campus Paris Diderot, irriguant le LIED par ses racines pluridisciplinaires. Deux autres modes l’ouvrent à des laboratoires d'autres PRES et aux entreprises, dont bon nombre le soutiennent très concrètement, en particulier celles qui font partie du conseil scientifique du LIED : AREVA, Bouygues, EDF, Saint-Gobain. Son ouverture internationale est le réseau PIERI.
La figure, généralisable à plusieurs fédérations de recherche, correspond au cas de deux : celle de l'institut des énergies de demain (avec l’UMR LIED représentée par un cercle blanc noté 1) et d’une autre centrée sur le laboratoire X (représenté par un cercle blanc noté X). Les laboratoires du campus Paris Diderot mutualisés avec le LIED sont les cercles colorés notés 2. Ceux satellisés par le X sont des cercles notés ? à colorer selon leurs disciplines. Les laboratoires interagissant avec le LIED et X sont les cercles colorés notés 3 ou 4 selon leurs degrés d’interaction avec ces laboratoires (sphères 3 ou 4 respectivement). Le caractère international du PIERI est symbolisé par un rectangle d’où proviennent des interactions multiformes.
Inscription au LIED
Researchers wishing to discuss collaborative projects are encouraged to get in touch using the following email address: lied-pieri@univ-paris-diderot.fr
Thèmes de recherche
The LIED and its international network PIERI (Paris Interdisciplinary Energy Research Institute) have been launched in the context of increased worldwide demands in energy in the face of environmental concerns, i.e. sustainable resources and impact on the climate.
The LIED favours a global approach and aims at resolving questions at the national and international level by a unique multi-disciplinary approach encompassing basic science (Biology, Chemistry, Physics, Informatics, Mathematics, Earth sciences and Engineering) as well as social sciences (Anthropology, Economics, Geography, History, Philosophy, Political sciences), whether in the academic or industrial domain.
· Objectives : Develop basic and applied science in response to the challenges of the “energy transition” with a focus on the interaction between energy, environment and climate. In the domain of biology, the LIED aims at understanding the basic mechanisms underlying energetic processes in microorganisms and in plants. It fosters applied science for the development of production and transformation of biomass.
· Methods : Biochemistry, Genetics, Genomics, Molecular and cellular biology, Bioinformatics.
. Research orientations : balancing fundamental and applied research.
The work of the PIERI is organised around four axes :
♦ Fundamental science and low-carbon energy sources ;
♦ The science and technology of energy efficiency ;
♦ Forecasting, social and economic analysis, and public policy studies ;
♦ Interdisciplinary epistemology.
The titles of first two axes already illustrate the aspiration to combine fundamental and applied research. Two complementary research groups have been established, entitled : ‘Innovative energy sources and biomaterials’ and ‘Energy transport, instabilities and fluctuations’, composed mainly of researchers from sciences and technologies, but with input from social scientists. The third axis is subdivided into six themes, which all bring together the physical and the social sciences, namely :
The geography of energy sourcing ;
The multilevel governance of energy ;
Social representation and innovation : hydrogen and other energy pathways ;
Energy efficiency and modes of consumption ;
Forecasting models and interdisciplinary convergence ;
Smart grids.
Equipes de recherche
Directeur : Mathieu Arnoux
Procédés des systèmes vivants
- Stress environnementaux et plantes : F. Bouteau ( MCF HDR, Coordinateur)
- Métabolisme secondaire des cyanobactéries : A. Mejean (PR, Coordinateur)
- Génétique et épigénétique des champignons : P. Silar (PR, Coordinateur)
- Biologie et Biotechnologie des champignons : F. Chapeland-Leclerc ( MCF HDR, Coordinateur)
Territoires et sociétés dans la longue durée
- Dynamiques des territoires et des sociétés : C. Mering (PR, Coordinateur), P. Chatzimpiros (MCF, Coordinateur)
- Dynamiques du long terme et transitions énergétiques : M. Arnoux (PR, Coordinateur)
- Sociologie et Sciences politiques : G. Bronner (PR, Coordinateur)
- Economie des inégalités : A.Berthe (MCF, coordinateur)
Efficience énergétique
- Dynamiques couplées et optimisation énergétique : C. Goupil (PR, Coordinateur)
- Dynamiques collectives des systèmes vivants et artificiels : J. Halloy (PR, Coordinateur)
- Climat Energie Métabolisme urbain : L. Royon (PR, Coordinateur)
[hal-01073863] Searching for a link between the L-BMAA neurotoxin and amyotrophic lateral sclerosis: a study protocol of the French BMAALS programme.
Date: 20 sep 2022 - 04:15
Desc: INTRODUCTION: Amyotrophic lateral sclerosis (ALS) is the most common motor neurone disease. It occurs in two forms: (1) familial cases, for which several genes have been identified and (2) sporadic cases, for which various hypotheses have been formulated. Notably, the β-N-methylamino-L-alanine (L-BMAA) toxin has been postulated to be involved in the occurrence of sporadic ALS. The objective of the French BMAALS programme is to study the putative link between L-BMAA and ALS. METHODS AND ANALYSIS: The programme covers the period from 1 January 2003 to 31 December 2011. Using multiple sources of ascertainment, all the incident ALS cases diagnosed during this period in the area under study (10 counties spread over three French regions) were collected. First, the standardised incidence ratio will be calculated for each municipality under concern. Then, by applying spatial clustering techniques, overincidence and underincidence zones of ALS will be sought. A case-control study, in the subpopulation living in the identified areas, will gather information about patients' occupations, leisure activities and lifestyle habits in order to assess potential risk factors to which they are or have been exposed. Specimens of drinking water, food and biological material (brain tissue) will be examined to assess the presence of L-BMAA in the environment and tissues of ALS cases and controls. ETHICS AND DISSEMINATION: The study has been reviewed and approved by the French ethical committee of the CPP SOOM IV (Comité de Protection des Personnes Sud-Ouest & Outre-Mer IV). The results will be published in peer-reviewed journals and presented at national and international conferences.
[hal-02173830] A gene graveyard in the genome of the fungus Podospora comata
Date: 13 sep 2022 - 13:46
Desc: Mechanisms involved in fine adaptation of fungi to their environment include differential gene regulation associated with single nucleotide polymorphisms and indels (including transposons), horizontal gene transfer, gene copy amplification, as well as pseudogenization and gene loss. The two Podospora genome sequences examined here emphasize the role of pseudogenization and gene loss, which have rarely been documented in fungi. Podospora comata is a species closely related to Podospora anserina, a fungus used as model in several laboratories. Comparison of the genome of P. comata with that of P. anserina, whose genome is available for over 10 years, should yield interesting data related to the modalities of genome evolution between these two closely related fungal species that thrive in the same types of biotopes, i.e., herbivore dung. Here, we present the genome sequence of the mat+isolate of the P. comata reference strain T. Comparison with the genome of the mat+isolate of P. anserina strain S confirms that P. anserina and P. comata are likely two different species that rarely interbreed in nature. Despite having a 94-99% of nucleotide identity in the syntenic regions of their genomes, the two species differ by nearly 10% of their gene contents. Comparison of the species-specific gene sets uncovered genes that could be responsible for the known physiological differences between the two species. Finally, we identified 428 and 811 pseudogenes (3.8 and 7.2% of the genes) in P. anserina and P. comata, respectively. Presence of high numbers of pseudogenes supports the notion that difference in gene contents is due to gene loss rather than horizontal gene transfers. We propose that the high frequency of pseudogenization leading to gene loss in P. anserina and P. comata accompanies specialization of these two fungi. Gene loss may be more prevalent during the evolution of other fungi than usually thought.
[cea-02341303] Metabolomic and proteomic investigations of impacts of titanium dioxide nanoparticles on Escherichia coli
Date: 13 sep 2022 - 13:46
Desc: The ecotoxicological impact of manufactured titanium oxide (TiO2) on Escherichia coli in a natural environmental medium, Seine River water, has been established through colony-forming unit technique and is closely related to the presence of little aggregates. Proteomic analyses by mass spectrometry and metabolomic analyses by NMR have been performed on water extracts. The influence of TiO2 concentration and ultraviolet irradiation on metabolomic profiles has been investigated. Processing metabolomics through Principal Components Analysis, together with an original method for processing proteomics data, revealed the up-regulation of proteins and the increase of fraction of metabolites related to energy and growth metabolism while other proteins are down-regulated and the fraction of other metabolites decreases. Moreover, the ATP content gradually rises in relation with the nano-TiO2 concentration in the medium, indicating a dramatic release of ATP by the damaged cells. These apparently contradictory results accredit the thesis of a heterogeneity of the bacterial population. This heterogeneity is also confirmed by Scanning Electron Microscopy images which show that while some bacteria are fully covered by nano-TiO2, the major part of the bacterial population remains free from nanoparticles, resulting in a difference of proteome and metabolome. The poster will particularly focus on the methodology used for metabolomics.
[hal-02178879] PaPro1 and IDC4, Two Genes Controlling Stationary Phase, Sexual Development and Cell Degeneration in Podospora anserina
Date: 13 sep 2022 - 13:46
Desc: Filamentous fungi frequently undergo bistable phenotypic switches. Crippled Growth of Podospora anserina is one such bistable switch, which seems to rely upon the mis-activation of a self-regulated PaMpk1 MAP kinase regulatory pathway. Here, we identify two new partners of this pathway: PaPro1, a transcription factor orthologous to Sordaria macrospora pro1 and Neurospora crassa ADV-1, and IDC4, a protein with an AIM24 domain. Both PaPro1 and IDC4 regulate stationary phase features, as described for the other actors of the PaMpk1 signaling pathway. However, PaPro1 is also involved in the control of fertilization by activating the transcription of the HMG8 and the mating type transcription factors, as well as the sexual pheromones and receptor genes. The roles of two components of the STRIPAK complex were also investigated by inactivating their encoding genes: PaPro22 and PaPro45. The mutants of these genes were found to have the same phenotypes as PaPro1 and IDC4 mutants as well as additional phenotypes including slow growth, abnormally shaped hyphae, pigment accumulation and blockage of the zygotic tissue development, indicating that the STRIPAK complex regulates, in addition to the PaMpk1 one, other pathways in P. anserina. Overall, the mutants of these four genes confirm the model by which Crippled Growth is due to the abnormal activation of the PaMpk1 MAP kinase cascade.
[cea-01532100] Metabolomic and proteomic investigations of impacts of titanium dioxide nanoparticles on Escherichia coli
Date: 13 sep 2022 - 13:46
Desc: In a previous study, it was demonstrated that the toxic impact of titanium dioxide nanoparticles on Escherichia coli starts at 10 ppm and is closely related to the presence of little aggregates. It was also assumed that only a part of the bacterial population is able to adapt to this stress and attempts to survive. Proteomic analyses, supported by results from metabolo-mics, reveal that exposure of $E. coli$ to nano-TiO$_2$ induces two main effects on bacterial metabolism: firstly, the up-regulation of proteins and the increase of metabolites related to energy and growth metabolism; secondly, the down-regulation of other proteins resulting in an increase of metabolites, particularly amino acids. Some proteins, e.g. chaperonin 1 or isocitrate dehydrogenase, and some metabolites, e.g. phenylalanine or valine, might be used as biomarkers of nanoparticles stress. Astonishingly, the ATP content gradually rises in relation with the nano-TiO$_2$ concentration in the medium, indicating a dramatic release of ATP by the damaged cells. These apparently contradictory results accredit the thesis of a heterogeneity of the bacterial population. This heterogeneity is also confirmed by SEM images which show that while some bacteria are fully covered by nano-TiO$_2$ , the major part of the bacterial population remains free from nanoparticles, resulting in a difference of prote-ome and metabolome. The use of combined–omics has allowed to better understand the heterogeneous bacterial response to nano-TiO$_2$ stress due to heterogeneous contacts between the protagonists under environmental conditions.
Autres contacts
Université Paris 7 - Paris Diderot
The members of LIED are located on 4 sites :
Bâtiment Condorcet - 10, rue Alice Domon & Léonie Duquet - 75013 Paris
Bâtiment Lamarck A - 39, rue Hélène Brion - 75013 Paris
Bâtiment Lamarck B - 35, rue Hélène Brion - 75013 Paris
Bâtiment Olympe de Gouges - 8 place Paul-Ricoeur - 75013 Paris
